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Extracellular vesicles : methods and protocols /

edited by Winston Patrick Kuo, Shidong Jia.

Book Cover
Names: Kuo, Winston Patrick, | Jia, Shidong,
Published: New York : Humana Press ; Springer, [2017]
Series: Methods in molecular biology (Clifton, N.J.) ; v. 1660.
Topics: Ribonucleases - Laboratory manuals. | Exosomes. | Secretory Vesicles. | Cell-Derived Microparticles.
Genres: Electronic books. | Laboratory manuals. | Laboratory Manuals.
Online Access: SpringerLink - Full text online
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245 00|aExtracellular vesicles :|bmethods and protocols /|cedited by Winston Patrick Kuo, Shidong Jia.
264 1|aNew York :|bHumana Press ;|bSpringer,|c[2017]
264 4|c©2017
300 |a1 online resource (xvi, 446 pages) :|billustrations (some color).
336 |atext|btxt|2rdacontent
337 |acomputer|bc|2rdamedia
338 |aonline resource|bcr|2rdacarrier
347 |atext file|bPDF|2rda
490 1 |aMethods in Molecular Biology,|x1064-3745 ;|vvolume 1660
504 |aIncludes bibliographical references and index.
505 00|tExtracellular vesicles : a brief overview and its role in precision medicine /|rMingyi Shang, John S. Ji, Chao Song, Bao Jun Gao, Jason Gang Jin, Winston Patrick Kuo, and Hongjun Kang --|tRed blood cells : a source of extracellular vesicles /|rWinston Patrick Kuo, John C. Tigges, Vasilis Toxavidis, and Ionita Ghiran --|tIsolation of extracellular vesicles by ultracentrifugation /|rFatemeh Momen-Heravi --|tSequential filtration : a gentle method for the isolation of functional extracellular vesicles /|rMitja L. Heinemann and Jody Vykoukal --|tPaper-based for isolation of extracellular vesicles /|rYi-Hsing Hsiao and Chihchen Chen --|tFilter-based extracellular vesicle mRNA isolation and high-throughput gene expression analysis /|rCindy M. Yamamoto, Taku Murakami, and Shu-Wing Ng --|tSpecific and generic isolation of extracellular vesicles with magnetic beads /|rKetil W. Pedersen, Bente Kierulf, and Axl Neurauter --|tPolymer-based purification of extracellular vesicles /|rPeter N. Brown and Hang Yin --|tSize exclusion chromatography : a simple and reliable method for exosome purification /|rRichard Lobb and Andreas Moller --|tPurification protocols for extracellular vesicles /|rRebecca E. Lane, Darren Korbie, Matt Trau, and Michelle M. Hill --|tCharacterization of extracellular vesicles by surface plasmon resonance /|rHyungsoon Im, Katherine Yang, Hakho Lee, and Cesar M. Castro --|tExtracellular vesicle isolation and analysis by western blotting /|rEmma J.K. Kowal, Dmitry Ter-Ovanesyan, Aviv Regev, and George M. Church --|tAnalysis of extracellular vesicles using fluorescence nanoparticle tracking analysis /|rPauline Carnell-Morris, Dionne Tannetta, Agnieszka Siupa, Patrick Hole, and Rebecca Dragovic --|tCharacterization of extracellular vesicles by flow cytometry /|rVirginia Camacho, Vasilis Toxavidis, and John C. Tigges --|tCharacterization of extracellular vesicles by size-exclusion high-performance liquid chromatography (HPLC) /|rTao Huang and Jiang He --|tMulti-surface antigen staining of larger extracellular vesicles /|rVeronika Lukacs-Kornek, Henrike Julich-Haertel, Sabine Katharina Urban, and Miroslaw Kornek --|tMicrocapillary chip-based extracellular vesicle profiling system /|rTakanori Akagi and Takanori Ichiki --|tDetection and characterization of extracellular vesicles by transmission and cryo-transmission electron microscopy /|rPetr Cizmar and Yuana Yuana --|tImaging of isolated extracellular vesicles using fluorescence microscopy /|rDmitry Ter-Ovanesyan, Emma J.K. Kowal, Aviv Regev, George M. Church, and Emanuele Cocucci --|tIn vivo tracking of extracellular vesicles in mice using fusion protein comprising lactadherin and Gaussia Luciferase /|rYuki Takahashi, Makiya Nishikawa, and Yoshinobu Takakura --|tTracking extracellular vesicles delivery and RNA translation using multiplexed reporters /|rAnthony Yan-Tang Wu and Charles Pin-Kuang Lai --|tExtraction and analysis of extracellular vesicle-associated miRNAs following antibody-based extracellular vesicle capture from plasma samples /|rDavide Zocco and Natasa Zarovni --|tExtracellular vesicle miRNA detection using molecular beacons /|rWon Jong Rhee and Seunga Jeong --|tRapid isolation of extracellular vesicles from blood plasma with size-exclusion chromatography followed by mass spectrometry-based proteomic profiling /|rSimion Kreimer and Alexander R. Ivanov --|tAdaptable polyethylene glycol-based workflow for proteomic analysis of extracellar vesicles /|rStephanie N. Hurwitz and David G. Meckes Jr. --|tProtocol for exosome isolation from small volume of ovarian follicular fluid : evaluation of ultracentrifugation and commercial kits /|rShlomit Kenigsberg, Brandon A. Wyse, Clifford L. Librach, and Juliano C. da Silveira --|tIsolation of extracellular vesicles in saliva using density gradient ultracentrifugation /|rKazuya Iwai, Satoshi Yamamoto, Mitsutaka Yoshida, and Kiyotaka Shiba --|tIsolation of extracellular vesicles from breast milk /|rXin Wang --|tIntegrated double-filtration microfluidic device for detection of extracellular vesicles from urine for bladder cancer diagnosis /|rLi-Guo Liang, Ye-Feng Sheng, Sherry Zhou, Fatih Inci, Lanjuan Li, Utkan Demirci, and ShuQi Wang --|tElectric field-induced disruption and releasing viable content from extracellular vesicles /|rChris Wang, Austin Wang, Fang Wei, David T.W. Wong, and Michael Tu --|tProduction and characterization of extracellular vesicles in malaria /|rSmart Mbagwu, Michael Walch, Luis Filgueira, and Pierre-Yves Mantel --|tIsolation of extracellular vesicles from stem cells /|rZixin Chen, Yongjun Li, Hong Yu, Yan Shen, Chengwei Ju, Genshan Ma, Yutao Liu, Il-man Kim, Neal L. Weintraub, and Yaoliang Tang --|tUse of peripheral extracellular vesicles for identification of molecular biomarkers in a solid tumor mouse model /|rNoemı Garcia-Romero, Gorjana Rackov, Cristobal Belda-Iniesta, and Angel Ayuso-Sacido --|tTherapeutic applications of extracellular vesicles : perspectives from newborn medicine /|rGareth R. Willis, Stella Kourembanas, and S. Alex Mitsialis --|tTherapeutic use of tumor cell-derived extracellular vesicles /|rJing Liu, Jingwei Ma, Ke Tang, and Bo Huang.
520 |aThis volume examines established methods and protocols to isolate and characterize extracellular vesicles (EVs) and their composition, among other techniques including purification, imaging, biofluid-specific and cell-specific isolation and downstream genomic and proteomic profiling. The international group of expert scientists who have contributed to this collection provide a variety of different techniques related to the growing assortment of EV applications, given that at times using only one technique or two is insufficient to address the question at hand. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Extracellular Vesicles: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of EV functions and applications.
588 0 |aOnline resource; title from PDF title page (SpringerProtocol, viewed August 22, 2017).
650 0|aRibonucleases|vLaboratory manuals.
650 7|aRibonucleases.|2fast|0(OCoLC)fst01097316
650 12|aExosomes.
650 2|aSecretory Vesicles.
650 22|aCell-Derived Microparticles.
655 4|aElectronic books.
655 7|aLaboratory manuals.|2fast|0(OCoLC)fst01920776
655 2|aLaboratory Manuals.
700 1 |aKuo, Winston Patrick,|eeditor.
700 1 |aJia, Shidong,|eeditor.
776 08|iPrint version:|z9781493972517
830 0|aMethods in molecular biology (Clifton, N.J.) ;|vv. 1660.|x1064-3745
856 40|3SpringerLink - Full text online|u|xUIU
938 |aYBP Library Services|bYANK|n14778024
994 |aC0|bUIU

Staff View for: Extracellular vesicles : methods and pro