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Germline stem cells : methods and protocols /

edited by Michael Buszczak.

Book Cover
Names: Buszczak, Michael.
Published: New York : Humana Press : Springer, ©2017.
Edition: 2nd edition
Series: Methods in molecular biology (Clifton, N.J.) ; v. 1463.
Springer protocols (Series)
Topics: Stem cells - Laboratory manuals. | Stem Cells.
Genres: Electronic books. | Laboratory manuals. | Laboratory manuals. | Laboratory Manuals.
Online Access: SpringerLink - Full text online
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245 00|aGermline stem cells :|bmethods and protocols /|cedited by Michael Buszczak.
250 |a2nd edition
264 1|aNew York :|bHumana Press :|bSpringer,|c©2017.
300 |a1 online resource (xi, 229 pages) :|billustrations (chiefly color).
336 |atext|btxt|2rdacontent
337 |acomputer|bc|2rdamedia
338 |aonline resource|bcr|2rdacarrier
490 1 |aMethods in molecular biology,|x1064-3745 ;|v1463
490 1 |aSpringer protocols
504 |aIncludes bibliographical references and index.
505 00|tAnalysis of the C. elegans germline stem cell pool /|rSarah L. Crittenden, Hannah S. Seidel, and Judith Kimble --|tMethods for studying the germline of the human parasite Schistosoma mansoni /|rJulie N.R. Collins and James J. Collins III --|tEvaluation of the asymmetric division of Drosophila male germline stem cells /|rMayu Inaba and Yukiko M. Yamashita --|tLive imaging of the Drosophila testis stem cell niche /|rLeah J. Greenspan and Erika L. Matunis --|tRNA isolation from early Drosophila larval ovaries /|rDana Gancz and Lilach Gilboa --|tLive-cell imaging of the adult Drosophila ovary using confocal microscopy /|rNevine A. Shalaby and Michael Buszczak --|tMeasurement of mRNA Poly(A) tail lengths in Drosophila female germ cells and germ-line stem cells /|rAymeric Chartier, Willy Joly, and Martine Simonelig --|tIdentification of germ-line stem cells in zebrafish /|rBruce W. Draper --|tPrimordial germ cell isolation from Xenopus laevis embryos /|rAmanda M. Butler [and others] --|tSingle-cell lineage analysis of oogenesis in mice /|rLei Lei and Allan C. Spradling --|tVisualization and lineage tracing of Pax7+ spermatogonial stem cells in the mouse /|rGina M. Aloisio [and others] --|tTransplantation as a quantitative assay to study mammalian male germline stem cells /|rAileen R. Helsel and Jon M. Oatley --|tMouse fetal germ cell isolation and culture techniques /|rCassy M. Spiller, Guillaume Burnet, and Josephine Bowles --|tRattus norvegicus spermatogenesis colony-forming assays /|rF. Kent Hamra --|tIdentification of mouse piRNA pathway components using anti-MIWI2 antibodies /|rTakamasa Hirano, Hidetoshi Hasuwa, and Haruhiko Siomi --|tEfficient induction and isolation of human primordial germ cell-like cells from competent human pluripotent stem cells /|rNaoko Irie and M. Azim Surani.
520 |aThis second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter's guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Germline Stem Cells: Second Edition aims to present the new findings and techniques that have developed since the first edition.
588 0 |aOnline resource; title from pdf of title page (SpringerProtocols, viewed October 14, 2016).
650 0|aStem cells|vLaboratory manuals.
650 7|aStem cells.|2fast|0(OCoLC)fst01132975
650 2|aStem Cells.
655 4|aElectronic books.
655 7|aLaboratory manuals.|2fast|0(OCoLC)fst01920776
655 7|aLaboratory manuals.|2lcgft
655 2|aLaboratory Manuals.
700 1 |aBuszczak, Michael.
776 08|iPrint version:|tCell microencapsulation.|dNew York : Humana Press, ©2017|z9781493940158
830 0|aMethods in molecular biology (Clifton, N.J.) ;|vv. 1463.
830 0|aSpringer protocols (Series)
856 40|3SpringerLink - Full text online|uhttp://www.library.illinois.edu/proxy/go.php?url=http://dx.doi.org/10.1007/978-1-4939-4017-2|xUIU
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Staff View for: Germline stem cells : methods and protoc