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Difference gel electrophoresis (DIGE) : methods and protocols /

edited by Rainer Cramer, Reiner Westermeier.

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Names: Cramer, Rainer. | Westermeier, Reiner.
Published: New York : Humana Press, ©2012.
Series: Methods in molecular biology (Clifton, N.J.) ; 854. 1064-3745.
Topics: Gel electrophoresis - Laboratory manuals. | Proteomics - Laboratory manuals. | Electrophoresis, Gel, Two-Dimensional. | Publication Formats | Biochemistry. | Genomics. | Investigative Techniques | Electrophoresis, Polyacrylamide Gel. | Genetics. | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Biological Science Disciplines. | Publication Characteristics | Computational Biology. | Electrophoresis. | Chemistry. | Chemistry Techniques, Analytical. | Natural Science Disciplines. | Biology. | Electrochemical Techniques. | Disciplines and Occupations | Two-Dimensional Difference Gel Electrophoresis. | Proteomics. | Laboratory Manuals | Methods.
Genres: Electronic books. | Laboratory manuals.
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245 00|aDifference gel electrophoresis (DIGE) :|bmethods and protocols /|cedited by Rainer Cramer, Reiner Westermeier.
260 |aNew York :|bHumana Press,|c©2012.
300 |a1 online resource (xiii, 401 pages)
336 |atext|btxt|2rdacontent
337 |acomputer|bc|2rdamedia
338 |aonline resource|bcr|2rdacarrier
347 |atext file|bPDF|2rda
490 1 |aMethods in molecular biology ;|vv. 854
500 |aIncludes index.
504 |aIncludes bibliographical references and index.
505 00|gPart I|tFundamentals --|tDIGE : past and future /|rJonathan S. Minden --|tBasics of 2D DIGE /|rPhil Beckett --|tMultifluorescence 2D gel imaging and image analysis /|rIngo Vormbrock, Sonja Hartwig, and Stefan Lehr --|tAssessing signal-to-noise in quantitative proteomics : multivariate statistical analysis in DIGE experiments /|rDavid B. Friedman --|tAnalysis of proteins using DIGE and MALDI mass spectrometry /|rWitold M. Winnik [and others] --|tSynthesis and validation of cyanine-based dyes for DIGE /|rMichael E. Jung [and others] --|gPart II|tMethods --|t2D DIGE saturation labeling for minute sample amounts /|rGeorg J. Arnold and Thomas Frohlich --|tProteomic analysis of redox-dependent changes using cysteine-labeling 2D DIGE /|rHong-Lin Chan, John Sinclair, and John F. Timms --|tAnalysis of protein posttranslational modifications using DIGE-based proteomics /|rRobert M. DeKroon [and others] --|tComparative analyses of protein complexes by blue native DIGE /|rKatrin Peters and Hans-Peter Braun --|t2D DIGE analysis of protein extracts from muscle tissue /|rCecilia Gelfi and Sara De Palma --|tCombination of highly efficient hexapeptide ligand library-based sample preparation with 2D DIGE for the analysis of the hidden human serum/plasma proteome /|rSonja Hartwig and Stefan Lehr --|t2D DIGE analysis of serum after fractionation by proteominertm beads /|rCynthia Liang, Gek San Tan, and Maxey C.M. Chung --|tStudy design in DIGE-based biomarker discovery /|rAlexandra Graf and Rudolf Oehler --|tComparative 2D DIGE analysis of the depleted serum proteome for biomarker discovery /|rMegan Penno, Matthias Ernst, and Peter Hoffmann --|gPart III|tApplications in clinical proteomics --|tDifferential gel-based proteomic approach for cancer biomarker discovery using human plasma /|rKeun Na [and others] --|t2D DIGE for the analysis of RAMOS cells subproteomes /|rMarisol Fernandez and Juan Pablo Albar --|tApplication of saturation labeling in lung cancer proteomics /|rGereon Poschmann [and others] --|tProteomic profiling of the epithelial-mesenchymal transition using 2D DIGE /|rRommel A. Mathias, Hong Ji, and Richard J. Simpson --|tMethod for protein subfractionation of cardiovascular tissues before DIGE analysis /|rAthanasios Didangelos, Xiaoke Yin, and Manual Mayr --|tApplication of DIGE and mass spectrometry in the study of type 2 diabetes mellitus mouse models /|rCelia Smith, Davinia Mills, and Rainer Cramer --|tEvaluating the efficacy of subcellular fractionation of blast cells using live cell labeling and 2D DIGE /|rYin Ying Ho [and others] --|gPart IV|tApplications in animal, plant, and microbial proteomics --|tDIGE analysis of plant tissue proteomes using a phenolic protein extraction method /|rChristina Rode [and others] --|tNative DIGE of fluorescent plant protein complexes /|rVeronika Reisinger and Lutz Andreas Eichacker --|tOverview of 2D DIGE analysis of marine (environmental) bacteria /|rRalf Rabus --|tApplication of 2D DIGE in animal proteomics /|rIngrid Miller.
520 |aProtein analysis is increasingly becoming a cornerstone in deciphering the molecular mechanisms of life. Proteomics, the large-scale and high-sensitivity analysis of proteins, is already pivotal to the new life sciences such as Systems Biology and Systems Medicine. Proteomics, however, relies heavily on the past and future advances of protein purification and analysis methods. DIGE, being able to quantify proteins in their intact form, is one of a few methods that can facilitate this type of analysis and still provide the protein isoforms in an MS-compatible state for further identification and characterization with high analytical sensitivity. Differential Gel Electrophoresis: Methods and Protocols introduces the concept of DIGE and its advantages in quantitative protein analysis. It provides detailed protocols and important notes on the practical aspects of DIGE with both generic and specific applications in the various areas of Quantitative Proteomics. Divided into four concise sections, this detailed volume opens with the basics of DIGE, the technique and its practical details with a focus on the planning of a DIGE experiment and its data analysis. The next section introduces various DIGE methods from those employed by scientists world-wide to more novel methods, providing a glance at what is on the horizon in the DIGE world. The volume closes with an overview of the wide range of DIGE applications from Clinical Proteomics to Animal, Plant, and Microbial Proteomics applications. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Differential Gel Electrophoresis: Methods and Protocols can be used by novices with some background in biochemistry or molecular biology as well as by experts in Proteomics who would like to deepen their understanding of DIGE and its employment in many hyphenations and application areas. With its many protocols, applications, and methodological variants, it is also a unique reference for all who seek fundamental details on the working principle of DIGE and ideas for possible future uses of DIGE in novel analytical approaches.
546 |aEnglish.
650 0|aGel electrophoresis|vLaboratory manuals.
650 0|aProteomics|vLaboratory manuals.
650 2|aElectrophoresis, Gel, Two-Dimensional.|0(DNLM)D015180
650 2|aPublication Formats
650 2|aBiochemistry.|0(DNLM)D001671
650 2|aGenomics.|0(DNLM)D023281
650 2|aInvestigative Techniques
650 2|aElectrophoresis, Polyacrylamide Gel.|0(DNLM)D004591
650 2|aGenetics.|0(DNLM)D005823
650 2|aAnalytical, Diagnostic and Therapeutic Techniques and Equipment
650 2|aBiological Science Disciplines.|0(DNLM)D001690
650 2|aPublication Characteristics
650 2|aComputational Biology.|0(DNLM)D019295
650 2|aElectrophoresis.|0(DNLM)D004586
650 2|aChemistry.|0(DNLM)D002621
650 2|aChemistry Techniques, Analytical.|0(DNLM)D002623
650 2|aNatural Science Disciplines.|0(DNLM)D010811
650 2|aBiology.|0(DNLM)D001695
650 2|aElectrochemical Techniques.|0(DNLM)D055664
650 2|aDisciplines and Occupations
650 2|aTwo-Dimensional Difference Gel Electrophoresis.|0(DNLM)D057825
650 2|aProteomics.|0(DNLM)D040901
650 2|aLaboratory Manuals
650 2|aMethods.|0(DNLM)D008722
650 7|aGel electrophoresis.|2fast|0(OCoLC)fst00939503
650 7|aProteomics.|2fast|0(OCoLC)fst01079785
655 4|aElectronic books.
655 7|aLaboratory manuals.|2fast|0(OCoLC)fst01920776
700 1 |aCramer, Rainer.
700 1 |aWestermeier, Reiner.
776 08|iPrinted edition:|z9781617795725
830 0|aMethods in molecular biology (Clifton, N.J.) ;|v854. 1064-3745.
852 8 |beresour-nc|hOnline Resource|t1|zAccessible anywhere on campus or with UIUC NetID
856 40|3SpringerLink - Full text online|u
938 |aYBP Library Services|bYANK|n7598525
994 |aC0|bUIU

Staff View for: Difference gel electrophoresis (DIGE) :