More Details for: Difference gel electrophoresis (DIGE) :

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Difference gel electrophoresis (DIGE) : methods and protocols /

edited by Rainer Cramer, Reiner Westermeier.

Book Cover
Names: Cramer, Rainer. | Westermeier, Reiner.
Published: New York : Humana Press, ©2012.
Series: Methods in molecular biology (Clifton, N.J.) ; 854. 1064-3745.
Topics: Gel electrophoresis - Laboratory manuals. | Proteomics - Laboratory manuals. | Electrophoresis, Gel, Two-Dimensional. | Publication Formats | Biochemistry. | Genomics. | Investigative Techniques | Electrophoresis, Polyacrylamide Gel. | Genetics. | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Biological Science Disciplines. | Publication Characteristics | Computational Biology. | Electrophoresis. | Chemistry. | Chemistry Techniques, Analytical. | Natural Science Disciplines. | Biology. | Electrochemical Techniques. | Disciplines and Occupations | Two-Dimensional Difference Gel Electrophoresis. | Proteomics. | Laboratory Manuals | Methods.
Genres: Electronic books. | Laboratory manuals.
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Physical Description: 1 online resource (xiii, 401 pages)
Includes: Includes bibliographical references and index.
ISBN: 9781617795732 (electronic bk.)
1617795739 (electronic bk.)

Language Note: English.
Summary: Protein analysis is increasingly becoming a cornerstone in deciphering the molecular mechanisms of life. Proteomics, the large-scale and high-sensitivity analysis of proteins, is already pivotal to the new life sciences such as Systems Biology and Systems Medicine. Proteomics, however, relies heavily on the past and future advances of protein purification and analysis methods. DIGE, being able to quantify proteins in their intact form, is one of a few methods that can facilitate this type of analysis and still provide the protein isoforms in an MS-compatible state for further identification and characterization with high analytical sensitivity. Differential Gel Electrophoresis: Methods and Protocols introduces the concept of DIGE and its advantages in quantitative protein analysis. It provides detailed protocols and important notes on the practical aspects of DIGE with both generic and specific applications in the various areas of Quantitative Proteomics. Divided into four concise sections, this detailed volume opens with the basics of DIGE, the technique and its practical details with a focus on the planning of a DIGE experiment and its data analysis. The next section introduces various DIGE methods from those employed by scientists world-wide to more novel methods, providing a glance at what is on the horizon in the DIGE world. The volume closes with an overview of the wide range of DIGE applications from Clinical Proteomics to Animal, Plant, and Microbial Proteomics applications. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Differential Gel Electrophoresis: Methods and Protocols can be used by novices with some background in biochemistry or molecular biology as well as by experts in Proteomics who would like to deepen their understanding of DIGE and its employment in many hyphenations and application areas. With its many protocols, applications, and methodological variants, it is also a unique reference for all who seek fundamental details on the working principle of DIGE and ideas for possible future uses of DIGE in novel analytical approaches.
Notes: Includes index.

More Details for: Difference gel electrophoresis (DIGE) :